Table 1.

14-3-3 and control GFP samples analyzed by MS

Sample - Prep #	Quantative analysis	Separation method	LC elution	MS method
1 GFP-prep1	n	2D-PAGE		MALDI Tof-Tof
2 14-3-3-prep1	n	2D-PAGE		MALDI Tof-Tof
3 GFP-prep2	n	1D-LC	ACN	ESI Ion-Trap
4 14-3-3-prep2	n	1D-LC	ACN	ESI Ion-Trap
5 14-3-3-prep3	n	n		n
6 GFP-prep3 (O18)	n	n		n
7 5 + 6	O16/O18	1D-LC	ACN	ESI Ion-Trap
8 GFP-prep4	n	2D-LC	A	MALDI Tof-Tof
9 GFP-prep5	n	2D-LC	B	MALDI Tof-Tof
10 14-3-3-prep4	n	2D-LC	A	MALDI Tof-Tof
11 14-3-3-prep5	n	2D-LC	B	MALDI Tof-Tof
12 14-3-3-prep5(O18)	n	2D-LC	B	MALDI Tof-Tof
13 9 + 12	O16/O18	2D-LC	B	MALDI Tof-Tof
14 GFP-prep6	n	n		n
15 14-3-3-prep6	n	n		n
16 14 + 15	iTRAQ	2D-LC	C	MALDI Tof-Tof
17 14-3-3-prep7	n	TiO2, 1D-LC	C	ESI Ion-Trap

a) Prep #: biological sample replicates

b) ACN: acetonitrile

c) ammonium acetate steps (mM): 20, 100, 500, 1000

d) ammonium acetate steps (mM): 20, 75, 250, 1000

e) ammonium acetate steps (mM): 0, 10, 25, 50, 75, 100, 150, 200, 250, 500

f) Any prep not particularly labeled O18 were O16-labeled

g) n: not applicable