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. 2014 Jul 1;9(7):e100947. doi: 10.1371/journal.pone.0100947

Figure 3. Chemical inhibition abolishes drug resistance of p53-null colon carcinoma cell lines.

Figure 3

A, C, E Lysates of HCT116p53KO (A) and SW480 (C) cells were harvested 24hs after treatment with 1 µM 6-bromoindirubin-3′-oxime (BIO) and 20 µM SB216763 (SB2) GSK3 inhibitors; lysates of HT-29 (E) cells were harvested 24 hs after treatment with 2 µM 6-bromoindirubin-3′-oxime (BIO) and 20 µM SB216763 (SB2) GSK3 inhibitors; specificity of the inhibitor for GSK3A was assessed by checking GSK3A activation/inactivation checked by western blot using a mix of pSer21-GSK3A and pSer9-GSK3B antibodies and antibody cross-reacting with both pTyr279-GSK3A and pTyr216-GSK3B. B, D, F) percentage of cell death of HCT116p53KO(B), SW480 (D) and HT-29 (F) treated with 200 µM 5FU in presence and in absence of the indicated inhibitors (72 hrs).