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. 2014 Jul 1;9(7):e101008. doi: 10.1371/journal.pone.0101008

Figure 3. UHPLC detection of zinniol in fungal extracts.

Figure 3

UHPLC chromatograms were obtained from different FRA017 Alternaria dauci fungal extracts and compared with an UHPLC chromatogram of pure synthetic zinniol. Retention times corresponding to main peaks are indicated A: UHPLC chromatogram of 10 µg synthetic zinniol. Observed zinniol retention time is 8.38 minutes B: UHPLC chromatogram of 13.4 µg organic extract of an A. dauci culture after 48 h under shaking conditions in carrot juice medium. Zinniol expected retention time of 8.38 minutes is indicated. C: UHPLC chromatogram of 13.4 µg organic extract of an A. dauci culture after 12 days without shaking (anoxic conditions) in V8 medium. A strong peak is visible, corresponding to zinniol retention time. D: UHPLC chromatogram of 13.4 µg organic extract of an A. dauci culture after 48 h under shaking conditions in V8 medium. Zinniol expected retention time of 8.38 minutes is indicated. Chromatograms C and D have the same scale. uAU: micro Absorption Units (optical density) at 233 nm.