Skip to main content
. 2014 Jul 1;9(7):e101008. doi: 10.1371/journal.pone.0101008

Table 2. Influence of cultivar, fungal exudate fractions and zinniol on cell suspension integrity and somatic embryogenesis.

Treatment Carrot genotype
Bolero H1 H4 I2 K3 Presto
rA1 ++2 − − +++ ++ −
rM ++ + + +++ ++ +
aA ++ ++ + +++ ++ +
aM ++ ++ + +++ ++ +
oA + − − ++ ++ −
oM ++ + + ++ ++ +
C ++ ++ + +++ ++ +
DMSO ++ + + +++ ++ +
z1 ++ + + +++ ++ +
z2 ++ + +/− +++ ++ +
z3 − − − + +/− −

Carrot cell suspensions with six different genotypes were tested for embryogenesis in the presence of fungal extracts and toxins. Embryogenesis was assessed 3 weeks after treatment.

1

Treatments were as follows: rA: Alternaria dauci (strain FRA017) fungal culture raw extract; rM: uninoculated medium raw extract; aA: A. dauci fungal culture aqueous extract; aM: uninoculated medium aqueous extract; oA: A. dauci fungal culture organic extract; oM: uninoculated medium organic extract; DMSO: DMSO solution, at a concentration corresponding to oM, z1, z2 and z3 treatments; z1: 0.025 µM zinniol; z2: 10 µM zinniol; z3: 500 µM zinniol. C: no treatment.

2

The signs are as follows: (−) no embryogenesis was visible and cells were damaged, (+) early-stage embryogenic masses were visible, (++) embryos were present, (+++) embryogenesis was profuse. +/− early-stage embryogenic masses were visible, or no embryogenesis was visible depending on the repetition.