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. 2014 Jul 1;9(7):e101010. doi: 10.1371/journal.pone.0101010

Figure 3. Effects of A279T on Wnt signaling and chemosensitivity in cancer cells.

Figure 3

*p<0.05, **p<0.01. A. TOP-flash promoter-reporter assay demonstrating that relative to wtTERT, A279T inhibits Wnt signaling in HeLa cells. This phenomenon was not seen in cells expressing G260D or A1062T. B. Immunoblot of β-catenin levels in cytoplasmic and nuclear extracts of EsC1 and EsC2 cells following constitutive expression of wtTERT or A279T-TERT. C. MTS assay demonstrating that relative to EsC1 and EsC2 cells expressing wtTERT, EsC1 and EsC2 cells expressing A279T are more sensitive to cisplatin (2 day treatment) and paclitaxel (3 hour treatment) measured on day 3.