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. 2014 Jul 1;9(7):e101173. doi: 10.1371/journal.pone.0101173

Figure 2. Binding of Stx holotoxins to glycolipid mixtures in absence of PC and Ch.

Figure 2

Binding was assessed by ELISA at 37°C using serial dilutions of Stx variants. A. Gb3; B. Gb3+Gal-Cer; C. Gb3+Glc-Cer; D. Gb3+Lac-Cer; E. Gb4; F. Gb4+Gal-Cer; Gb4+Glc-Cer; Gb4+Lac-Cer. Mixtures of glycolipids were prepared in methanol in the ratio of 1∶1 of the two glycolipids. Total concentration of 200 ng glycolipid was added per well. As negative control toxins were incubated with plate sham-coated with methanol. In all experiments, background RFU values obtained in methanol were subtracted from each value. The RFU signal is the mean of three independent experiments and error bars indicate standard deviation (SD).