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. 2014 Jul 1;9(7):e101173. doi: 10.1371/journal.pone.0101173

Figure 3. Binding of Stx holotoxins to glycolipid mixtures in presence of PC and Ch.

Figure 3

Binding was assessed by ELISA at 37°C using serial dilutions of Stx variants. A. Gb3; B. Gb3+Gal−Cer; C. Gb3+Glc−Cer; D. Gb3+Lac−Cer; E. Gb4; F. Gb4+Gal−Cer; Gb4+Glc−Cer; Gb4+Lac−Cer. Mixtures of glycolipid 1, glycolipid 2, PC and Ch were prepared in ratio of 1∶1∶3∶3, respectively, to make 200 ng of total glycolipid concentration per well. Binding was assessed as described in Figure 2. The RFU signal is the mean of three independent experiments and error bars indicate SD.