. 2014 Jul 1;9(7):e101173. doi: 10.1371/journal.pone.0101173

Table 4. EC₅₀ values (in), glycolipid binding dissociation constants for Stx holotoxin and B-subunits (N.D.: Not determined due to insignificant binding).

	Glycolipid Binding, K_D in μM (Hill coefficient)				AUC[46]
	Gb3+PC+Ch	Gb4+PC+Ch	Gb3	Gb4	EC₅₀ (μM)
Holotoxin
Stx1	0.046 (1)	0.105 (1)	0.139 (1.2)	0.308 (1.2)	-
Stx2a	0.025 (0.7)	0.035 (0.8)	0.074 (0.8)	N.D.	-
Stx2b	0.094 (0.8)	N.D.	0.308 (0.7)	N.D.	-
Stx2c	0.210 (0.7)	0.915 (0.8)	0.192 (0.8)	N.D.	-
Stx2d	0.032 (0.9)	0.653 (0.9)	N.D.	N.D.	-
B-subunits
Stx1	0.018 (1)	0.011 (1.1)	0.027 (1)	0.026 (1.1)	0.043
Stx1-L41Q	3.372 (1.6)	2.329 (2.1)	2.234 (2)	2.584 (2.1)	1.060
Stx2a	0.141 (1.6)	0.235 (1.4)	0.418 (2.7)	0.559 (2.2)	2.290
Stx2a-Q40L	0.003 (0.8)	0.005 (1.0)	0.005 (0.7)	0.128 (0.8)	0.693
Stx2c	0.583 (1.6)	0.453 (1.6)	0.117 (1.2)	0.778 (1.9)	-
Stx2d	0.176 (1.5)	0.243 (1.5)	0.278 (2.6)	0.423 (2)	-

Table 4. EC50 values (in), glycolipid binding dissociation constants for Stx holotoxin and B-subunits (N.D.: Not determined due to insignificant binding).