Figure 1. Normal fertility in oocyte STAT3 mutant Stat3^fl/fl;Zp3-cre⁺mice.

Reproductive ability of the Stat3;ZP3Cre line was tracked over a 26 week breeding period. (A) Cumulative number of pups (asterix indicates statistical significance from other groups, P < 0.05, one way ANOVA and Tukey's post hoc test). (B) average number of litters per 30 day period. (C) average number of pups per litter. (n = 5 breeder pairs per genotype). (D) Recombination of the Stat3 gene in COCs was confirmed by PCR with the amplification of a 310 bp truncated product in heterozygous (Stat3^fl/+; Zp3-cre⁺) and knock out (Stat3^fl/fl;Zp3-cre⁺) mice. (E) STAT3 immuno staining (brown) in ovaries from mice at 26 days old, bar  = 500 µm.