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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: Regen Med. 2014 Mar 12;9(5):593–607. doi: 10.2217/rme.14.17

Figure 1. Phenotypic and functional characterization of cells grown from Osteocel® explant cultures.

Figure 1

(A) Proliferative and (B) differentiation capacities, chondrogenesis and adipogenesis were assessed on day 21 post-induction by Toluidine Blue or Oil Red-O staining, respectively. Osteogenesis was assessed by alkaline phosphatase staining on day 14 post-induction; original magnification of microphotographs ×100 and ×40 for chondrogenesis. (C) Phenotypic characterization of Osteocel-derived cells confirming their identity as multipotential stromal cells (MSCs; n = 3 Osteocel lots). Gray filled histograms indicate isotype control staining. (D-F) Immunosuppressive capacity of Osteocel-MSCs in co-culture with T cell responders (Tresp) CD4+ T cells. (D) Gating strategy for Tresp cell employing initial gating for live cells followed by exclusion of MSCs and final gating on the CD4+ population. (E) Histograms indicating proliferation patterns of Tresp co-cultured at ratios 1:1 to 1:4 with MSCs (a representative donor, colors represent cell doublings). (F) Inhibition of Tresp proliferation measured as relative division index normalized to no MSC control (n = 4 Osteocel lots, 1:1 to 1:8 ratios). Error bars represent SD.

FI: Fluorescence intensity; MSC: Multipotential stromal cell; SSC: Side scatter.