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. Author manuscript; available in PMC: 2014 Nov 6.
Published in final edited form as: Regen Med. 2014 Mar 12;9(5):593–607. doi: 10.2217/rme.14.17

Figure 4. Analysis of CD45−CD271+ multipotential stromal cell abundance in femoral head bone digests.

Figure 4

(A) New live/dead binding dyes compared with (B) a traditional 7-AAD-based method. Far left panels: dot plots showing size distribution of all events (cells and debris). Middle left panels: gating on live cells (shown in red). Middle right panels: identification of CD45−CD271+ MSCs (shown in blue). Far right panels: histogram of CD73 positivity on the CD45−CD271+ MSC population. Vertical lines indicate the boundary for isotype control binding, horizontal lines indicate the region used to calculate the proportions of CD73+ cells.

MSC: Multipotential stromal cell; FSC: Forward scatter; SSC: Side scatter.