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Genotyping of F2, F5, F12, MTHFR, and HFE by melting curves.
Notes: We compared the melting curves for several genes. The protocol without DNA extraction proved to be equivalent, in terms of efficiency, when performed in both single and/or multiplex (duplex or triplex) reactions.
Abbreviations: −(d/dT), negative derivative of fluorescence over temperature; −/−, normal; +/−, heterozygous; +/+, homozygous.
