Table 3.
Reaction mix used for RT-PCR procedure
| Reaction mix | MTHFR (single) | HFE (H63D) (single) | HFE (C282Y) (single) |
|---|---|---|---|
| Mix hybridization probes (no Plus) 10× | 2 μL | 2 μL | 2 μL |
| H2O | 8.4 μL | 0.2 μL | 8.4 μL |
| MgCl2 | 1.6 μL | 0.8 μL | 1.6 μL |
| MTHFR Fw (4 μM) | 1 μL | – | – |
| MTHFR Rv (4 μM) | 3 μL | – | – |
| MTHFR-Flu (4 μM) | 1 μL | – | – |
| MTHFR-640 (4 μM) | 1 μL | – | – |
| H63D Fw (4 μM) | – | 1 μL | – |
| H63D Rv (4 μM) | – | 3 μL | – |
| H63D-Flu (4 μM) | – | 1 μL | – |
| H63D-610 (4 μM) | – | 1 μL | – |
| F12 Fw (4 μM) | – | – | 1 μL |
| F12 Rv (4 μM) | – | – | 3 μL |
| F12-Flu (4 μM) | – | – | 1 μL |
| F12-640 (4 μM) | – | – | 1 μL |
| WBCs (5×106 WBC/mL) | 2 μL | 2 μL | 2 μL |
Abbreviations: WBCs, white blood cells; RT-PCR, real-time polymerase chain reaction.