Table 2. Bioinformatics pathway analysis to predict molecular functions and biological processes significantly affected by the loss of Scx in heart valves at E15.5.
| Molecular function/Biological process | Bioinformatics Source and Associated Pathway Number | p-value | Gene | Fold Change |
| Lysine N-methyltransferase activity | GO:0016278 | 0.0005 | Dot1l | 0.66 |
| Histone lysine N-methyltransferase | GO:0018024 | 0.0005 | Mll2 | 0.52 |
| Protein lysine N-methyltransferase activity | GO:0016279 | 0.0005 | Wbp7 | 0.54 |
| Histone methyltransferase activity | GO:0042056 | 0.0009 | ||
| Protein methyltransferase activity | GO:0008276 | 0.0022 | ||
| N-methyltransferase activity | GO:0008170 | 0.0017 | ||
| S-adenosylmethionine-dependent methyltransferase activity | GO:0008757 | 0.0058 | ||
| Transcription regulatory region DNA binding | GO:0044212 | 0.0071 | Junb | 0.49 |
| Regulatory region nucleic acid binding | GO:0001067 | 0.0071 | Hlx | 0.42 |
| Regulatory region DNA binding | GO:0000975 | 0.0071 | Nfatc2 | 0.53 |
| Ncor2 | 0.46 | |||
| Mll2 | 0.52 | |||
| Notch binding | GO:0005112 | 0.0007 | Dll4 | 0.61 |
| Ncor2 | 0.46 | |||
| 9-cis-retinoic acid biosynthesis process | GO:0042904 | 0.00006 | Aldh1a1 | 2.29 |
| 9-cis-retinoic acid metabolic process | GO:0042905 | 0.00006 | Aldh1a2 | 1.52 |
| Vitamin A biosynthetic process | GO:0035238 | 0.00006 | ||
| Retinoic acid biosynthetic process | GO:0002138 | 0.00020 | ||
| Diterpenoid biosynthetic process | GO.0016102 | 0.00020 | ||
| Terpenoid biosynthetic process | GO:0016114 | 0.00040 | ||
| Fat-soluble vitamin biosynthetic process | GO:0042363 | 0.00009 | ||
| Retinoic acid metabolic process | GO:0042573 | 0.00130 | ||
| 3-chloroallyl aldehyde dehydrogenase activity | GO:0004028 | 0.00040 | ||
| Retinal dehydrogenase activity | GO:0001758 | 0.00040 | ||
| Nucleosomal DNA binding | GO:0031492 | 0.0006 | Hmgn5 | 1.60 |
| Hmgn3 | 1.61 | |||
| Secondary active transmembrane transporter activity | GO:0015291 | 0.0122 | AI317395 | 0.64 |
| Slc9a8 | 0.66 | |||
| Slc16a4 | 0.54 | |||
| Cysteine and methionine metabolism | KEGG:00270 | 0.0027 | Mpst | 1.74 |
| Trdmt1 | 1.59 | |||
| Cdo1 | 1.64 | |||
| Pentose and glucuronate interconversions | KEGG:00040 | 0.0079 | Akr1b7 | 5.76 |
| Aldh1a1 | 2.29 | |||
| Notch Signaling Pathway | KEGG:04330 | 0.0090 | Dll4 | 0.61 |
| Ncor2 | 0.46 | |||
| Maml1 | 0.64 | |||
| Notch Signaling Pathway | Wiki | 0.0010 | Dll4 | 0.61 |
| Ncor2 | 0.46 | |||
| Maml1 | 0.64 | |||
| Wiki | 0.0146 | Dll4 | 0.61 | |
| Delta-Notch Signaling Pathway | Ncor2 | 0.46 | ||
| Maml1 | 0.64 | |||
| Cellular Development | Ingenuity: IPA | 3.26E-04 | Fam20c | 0.57 |
| Fas | 1.76 | |||
| Gpc4 | 0.52 | |||
| Hspg2 | 0.55 | |||
| Junb | 0.56 | |||
| Lrp5 | 0.49 | |||
| Maml1 | 0.56 | |||
| Ncor2 | 0.64 | |||
| Nfatc2 | 0.46 | |||
| Nod1 | 0.53 | |||
| Nppc | 0.51 | |||
| Nucb2 | 3.10 | |||
| Cell Morphology | Ingenuity: IPA | 4.52E-04 | Efna5 | 0.55 |
| Fas | 1.76 | |||
| Cellular Assembly and Organization | Ingenuity: IPA | 4.52E-04 | Fas | 1.76 |
| Plec | 0.66 | |||
| Cellular Compromise | Ingenuity: IPA | 4.52E-04 | Fas | 1.76 |
| Plec | 0.66 | |||
| Cell Death and Survival | Ingenuity: IPA | 1.25E-03 | Maml1 | 0.64 |
| Plec | 0.66 |
(GO, Gene Ontology).