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. 2014 Jul 1;9(7):e101453. doi: 10.1371/journal.pone.0101453

Figure 1. Assessment of the pre-storage confounding factors.

Figure 1

(A) CSF samples were frozen under different conditions. Box-plots report the median activity, 25–75 percentiles and ranges. * = p<0.05; ** = p<0.01; *** = p<0.001. (B,C) CSF aliquots were kept at 4°C (B) or 22°C (C) for 1, 4, 24 or 48 hours before freezing. The results are reported as the mean percentage activity change ± the SD with respect to fresh CSF. * = at least p<0.05. α-man: α-mannosidase; α–fuc: α-fucosidase; β-man: β-mannosidase; β-gal: β-galactosidase; GCase: β-glucocerebrosidase; β-hex: β-hexosaminidase; CatD: cathepsin D; CatE: cathepsin E.