Table 6. Relative percentage change of lysosomal enzymes activities during 5 freeze/thaw (F/T) cycles.

Enzyme	C1	C2	C3	C4	C5
α-mannosidase	−18.18±2.90**	−21.55±7.30**	−23.02±8.53**	−34.31±11.76***	−45.63±6.73***
α-fucosidase	−2.22±4.67	21.25±16.72*	27.51±12.29**	19.45±9.26*	5.13±13.31
β-mannosidase	−3.91±7.07	32.18±20.11**	47.18±15.83**	44.65±18.33**	29.45±24.55**
β-galactosidase	3.00±4.21	10.9±13.92	6.76±10.96	0.78±15.55	−15.02±11.77*
GCase	−7.3±4.64	−14.68±12.82	−17.93±11.79	−22.82±13.76	−45.42±5.23**
β-hexosaminidase	−3.85±3.94	25.65±6.68*	34.74±9.38**	31.39±12.02**	−6.98±16.16
cathepsin D	−1.55±1.46	0.59±14.63	−6.24±5.20	−5.06±5.66	−10.25±4.96*
cathepsin E	−1.51±3.88	3.82±6.96	0.85±10.12	3.13±8.80	1.16±8.53

The percentage change of all enzymes activity tested across five F/T cycles is reported.

* = p<0.05;

**p<0.01;

*** p<0.001.