Table 1. Primers and amplification conditions used for RT-PCR.
Gene | accession | primer | product | annealing |
GLO1 | NM_006708.2 | TGACCATTGTGCTCTTGGCT AGTACAAGCACGGTTGGCAT | 395 bp | 52°C |
GLO2 (HAGH) | NM_005326.4 NM_001040427.1 | CCAGCCCTGCTGGGAGTT CGACTCCAGCTTGACCAGTT | 279 bp | 52°C |
FN3K | NM_022158.3 | CATCCCGCAGGTGAATGAGT GAAGGAAGCCGGGTCGTAAA | 256 bp | 52°C |
VEGF var. 1-9 | NM_001025366.2 | GGGCAGAATCATCACGAAGT TGGTGATGTTGGACTCCTCA | 211 bp | 58°C |
GAPDH | NM_002046.4 NM_001256799.1 | ATCATCCCTGCCTCTACTGG CCCTCCGACGCCTGCTTCAC | 188 bp | 57°C |
α-tubulin | NM_032704.3 NM_006082.2 | TCTTCAGGGCTTCTTGGTTT GGTGGTGAGGATGGAGTTGT | 181 bp | 56°C |