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. 2014 Jul 1;9(7):e101473. doi: 10.1371/journal.pone.0101473

Table 1. Primers and amplification conditions used for RT-PCR.

Gene accession primer product annealing
GLO1 NM_006708.2 TGACCATTGTGCTCTTGGCT AGTACAAGCACGGTTGGCAT 395 bp 52°C
GLO2 (HAGH) NM_005326.4 NM_001040427.1 CCAGCCCTGCTGGGAGTT CGACTCCAGCTTGACCAGTT 279 bp 52°C
FN3K NM_022158.3 CATCCCGCAGGTGAATGAGT GAAGGAAGCCGGGTCGTAAA 256 bp 52°C
VEGF var. 1-9 NM_001025366.2 GGGCAGAATCATCACGAAGT TGGTGATGTTGGACTCCTCA 211 bp 58°C
GAPDH NM_002046.4 NM_001256799.1 ATCATCCCTGCCTCTACTGG CCCTCCGACGCCTGCTTCAC 188 bp 57°C
α-tubulin NM_032704.3 NM_006082.2 TCTTCAGGGCTTCTTGGTTT GGTGGTGAGGATGGAGTTGT 181 bp 56°C