Table 2. Relative mRNA accumulation of genes involved in dicarbonyl defence and hypoxia.

Genes	MCF-7 normoxia	TamR normoxia	MCF-7 hypoxia	TamR hypoxia
GLO1	1.0+/−0.14	1.59+/−0.30	2.03+/−0.36 $	1.11+/−0.24
GLO2	1.0+/−0.15	0.97+/−0.16	1.50+/−0.19	0.79+/−0.28
FN3K	1.0+/−0.19	0.55+/−0.10	1.16+/−0.37	1.36+/−0.28 $
VEGF	1.0+/−0.08	0.75+/−0.18	91.9+/−30.1 *	24.0+/−5.9 * §
GAPDH	1.0+/−0.07	0.96+/−0.15	7.44+/−1.13 *	6.68+/−1.27 *

Cells were cultivated for 24 h under normoxic or hypoxic conditions and mRNA accumulation determined by qRT-PCR as described in material and methods. mRNA accumulation was normalized towards α-tubulin mRNA content and MCF-7 under normoxic conditions was set to 1. Average and standard error (SEM) are given. Experiment was performed in triplicate with each data point determined with six repetitions each.

Statistics (one way ANOVA, Tamhane T2, post hoc analysis):

*: p<0.05 versus same cell line control.

^$: p<0.1 versus same cell line control.

^§: p = 0.1 versus other cell line, same treatment.