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. 2014 Jul 1;9(7):e101457. doi: 10.1371/journal.pone.0101457

Figure 5. Effects of miR-204 on SOX4 expression by luciferase reporter assay.

Figure 5

The potential miR-204 binding site at the 3′-UTR of SOX4 mRNA was computationally predicted by Targetscan. Cells were co-transfected with miR-204 mimics (or negative control) with pGL3-SOX4 (or pGL3-SOX4-mut) vector. Luciferase activity was normalized by the ratio of firefly and Renilla luciferase signals. * indicates P<0.05.