Table 1. EagI and AscI restriction sites.
| Gene name | Primers (5′-3′) |
|---|---|
| Melan-A |
F: CCCTACAAGCGGCCGAATGCCAAGAGAAG |
| R: CGCTGGCTCTTAGGCGCGCCAGGTGAATAAGG |
|
| gp100 |
F: GAAGAACACACGGCCGAAATGGATCTGGTGC |
| R: TGAGAGTACTCAGGCGCGCCGACCTGCTG |
|
| tyrosinase |
F: AGAGGAAGACGGCCGAATGCTCCTGGCTGTT |
| R: TGCCTAAGCCTTTTAGGCGCGCCTAAATGGCTCTG |
|
| TRP1 |
F: TCAAGCAGACGGCCGAATGAGTGCTCCTAAA |
| R: GGGCATTTGTTAGGCGCGCCGACCACAGACTG |
|
| TRP2 |
F: TATAAAGCCCGGCCGAATGAGCCCCCTTTGG |
| R: ATGAGCACCCTAGGCGCGCCGGCTTCTTCTGT |
|
| KU-MEL-1 |
F: ACAGTTCAATCGGCCGAATGGGGGACATTC |
| R: AGCCCTGACTTAGGCGCGCCCACTGGGTA |
|
| MAGEA3 |
F: ACCAGAGTCATCGGCCGCATGCCTCTTG |
| R: CTCAGACTCAGGCGCGCCCTCTTCCCCC |