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. 2014 Jun 25;7:1159–1168. doi: 10.2147/OTT.S62158

Figure 4.

Figure 4

Effect of GSK-3 inhibition on cell viability.

Notes: LiCl (A and B) and TDZD-8 (C and D) reduces viability of ovarian carcinoma cells; SKOV3 (A and C) and SKOV3-TR30 (B and D) cells were treated with various concentrations of LiCl or TDZD-8, and cell viability was determined using the MTT assay at 24, 48, and 72 hours; Data represent the mean of three different experiments with triplicate wells; To determine GSK-3α/β protein levels after siRNA treatment, protein extracts were obtained using a lysis buffer containing protease inhibitors cocktail 72 hours posttransfection and analyzed by Western blot (E); A reduction of ≥70% in the expression levels of the corresponding GSK-3 isoforms was observed when compared to untransfected or scrambled siRNA transfected controls. GSK-3 silencing decreases viability of ovarian carcinoma cells (F); Cell viability was determined by MTT assay 72 hours after transfection with GSK-3α siRNA (70 nM), GSK-3β siRNA (50 nM), or both in SKOV3 and SKOV3-TR30 cells; Data represent the mean of three different experiments with triplicate wells. *P<0.05, #P<0.01 compared with control siRNA.

Abbreviations: GSK, glycogen synthase kinase; LiCl, lithium chloride; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; siRNA, small interfering RNA; TDZD-8, 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione.