Table 1. Primers used to isolate a degenerate fragment of Awi-GABARAPL2, and to perform 5′ and 3′ RACE reactions.
Primer name | Primer sequence (5′ to 3′) | Translation |
---|---|---|
ATG8 degenerate forward | GARAARGCMATGTTYYTGTTTGT | EKAMFLFV |
ATG8 degenerate reverse | CCRAAWGTRTTYTCTCCRCTGTAAG | AYSGENTFG |
Awi-GABARAPL2-specific forward | ACGTGATAATTGCCGAGCGG | located in 5′ UTR |
Awi-GABARAPL2-specific reverse | GTTCTGGGGAAAAGGTCCAGTGGT | located in 3′ UTR |