Figure 3.atg17 is necessary for developmental autophagy and proper salivary gland histolysis. (A and B) Punctate LTR staining is observed in controls (A), but not in atg17-null mutant fat bodies of L3 stage wandering larvae prior to metamorphosis (B). (C) Quantification of data from (A and B); n = 20/genotype. (D) Depletion of atg17 in GFP-positive cells blocks the formation of mCherry-Atg8a positive autophagosomes and autolysosomes in fat bodies of wandering larvae. (E) Quantification of data from (D); n = 26. (F–I) Larval salivary glands (SG) are visible in hematoxylin-eosin stained paraffin sections of both control (F, n = 3) and atg17-null mutant (G, n = 5) white prepupae (0 h relative to puparium formation, rpf). Salivary gland histolysis is completed by 24 h rpf in controls (H, n = 7), but numerous large, persisting salivary gland cells (arrowheads) are visible in all similarly aged atg17-null mutant pupae (I, n = 9). Scale bars: (A, B, and D) 20 µm, (F–I) 60 µm. Error bars: s.d., **P < 0.01.