Figure 4.MIR142-3p targets the ATG16L1 3′UTR. (A) HCT116 cells were cotransfected with the F1 vector (200 ng/ml) and a miRNA mimic (10 nM), and luciferase reporter assays were performed 48 h after transfection. The data are expressed as the mean ± SEM (n = 4). **P < 0.01 vs the MIRNC. (B) To validate the predicted MIR142-3p binding sequence in the ATG16L1 3′UTR, 11 nucleotides at position 254 to 264 in the truncated F1 vector were substituted. (C) HCT116 cells were cotransfected with the WT or mutated F1 (200 ng/ml) vector and the MIR142-3p mimic or MIRNC (10 nM). At 48 h post-transfection, dual luciferase activities were determined. The data are expressed as the mean ± SEM (n = 4). *P < 0.05 and ***P < 0.001 vs the MIRNC.