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The impact of C35-PP on bacitracin sensitivity is affected by the expression level of UppS. (A) Bacitracin disk diffusion assays for WT (168 trpC2), uppS1 (HB13648), ΔytpB (HB13321), and uppS1 ΔytpB (HB13443) strains on MH medium; (B) Bacitracin disk diffusion assays illustrating the loss of the ZOEL in a strain overexpressing UppS (ΔytpB Pspac(hy)-uppS (HB17057; right) as compared to the isogenic ΔytpB (HB13350) strain (left) when grown on MH medium containing 1 mM IPTG.
