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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Hepatology. 2014 May 28;60(1):278–289. doi: 10.1002/hep.27084

Figure 1. Sox9 clonal lineage tracing identifies ductal progenitors but not hepatocytes in homeostasis.

Figure 1

(a) Sox9-CreERT2 R26R-Confetti+/- mice dosed with tamoxifen at limiting dilutions produced recombination in ductal progenitor cells (CD45- CD31- MIC1-1C3+) (b) FACS sorted MIC1-1C3+, Sox9-CreERT2 Confetti+ marked single cells initiated self-renewing organoids (YFP sorted) after 10 days culture or (c) 14 days (YFP+, mCerulean+, or RFP+ sorter) (d) Clonally labeled organoids self-renewed after passage. (e) Sox9-CreERT2 clonally marked cells continued to express ductal marker Opn after 3 months of homeostasis (scale bars = 50μm).