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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Hepatology. 2014 May 28;60(1):278–289. doi: 10.1002/hep.27084

Figure 2. Sox9+ ducts rarely give rise to hepatocytes in CDE injury.

Figure 2

(a) Experimental scheme: 4-6 weeks old Sox9-CreERT2 R26R-Confetti+/- mice were given a single dose of tamoxifen followed by oval cell injury two weeks later (b) CDE diet produced ductal proliferation after low density Sox9-labeling. A Z-projection generated from confocal analysis of 100 μ m thick liver section (32mg/kg tamoxifen). Ductal proliferation was not associated with hepatocyte-differentiation (c) Immunostaining for ductal markers A6 (top), Hnf4a (bottom) confirmed Sox9-CreERT2 marked cells did not differentiate into hepatocytes after recovery from CDE injury. (d) 2 YFP+ cells with distinct hepatocyte morphology (arrowhead) were adjacent to clonally unrelated mCerulean+ and RFP+ cholangiocytes (arrows). Scale bars = 50μm.