Figure 3. Sox9+ ducts do not differentiate into hepatocytes in DDC or CCl4 injury.
(a) Immunofluorescence for ductal marker Opn (top) and hepatocyte marker Hnf4a (bottom) showed Sox9-CreERT2 marked cells retained ductal fate after regeneration from 5 weeks CCl4 injury (arrows indicate a unique clone). (b) Immunofluorescence for ductal markers A6 (top) and Opn (bottom) continued to co-localize with Sox9-CreERT2 marked clones after 4 weeks of DDC injury. Scale bars = 50μm.