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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Hepatology. 2014 May 28;60(1):278–289. doi: 10.1002/hep.27084

Figure 4. Robust and specific replacement of adult hepatocytes but not other cells in Fah-chimeric mice.

Figure 4

(a) Experimental scheme: gravity enriched wildtype or mTomatoFah+/+ hepatocytes were transplanted into the spleen of Fah-/- mice and allowed to repopulate (95-99%).

(b) Fah-immunostaining (brown) shows >99% of hepatocytes were donor derived after 10 weeks repopulation (bar = 1mm) (c) Complete central vein (CV) to portal vein (PV) repopulation with donor Fah+ hepatocytes (brown). (d) Donor cell marker (mTomato red) did not colocalize with Osteopontin+ (green) host duct cells. (e) Organoids (arrows) formed from dissociated chimeric liver were host derived (mTomato negative). mTomato+ hepatocytes did not form organoid spheres after 12 days culture.