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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Hepatology. 2014 May 28;60(1):278–289. doi: 10.1002/hep.27084

Figure 6. Mature hepatocytes self-renew in oval cell injuries.

Figure 6

(a) Experimental scheme: Hepatocytes were marked at low density in heterozygous R26R-Confetti+/- mice using low dose rAAV8-Ttr-Cre. Partial hepatectomy was performed and injuries were initiated after complete regeneration. (b) Prior to hepatectomy single hepatocytes were marked. (c) Partial hepatectomy induced hepatocyte replication producing clones of 1 (single arrow) or two identically marked cells (double arrow). (d) Oval cell injury with CDE diet, DDC diet, or chronic carbon tetrachloride induced mature hepatocyte replication and self-renewal or clonal expansion. (e) Dot plot histogram of mature hepatocyte colony size at baseline, partial hepatectomy followed by 2 weeks to 6 months homeostasis, and after oval cell injury (line = mean ± SD). Scale bars = 100μm.