Fig. 1.

Contribution of MesP1+ mesoderm to HSCs, PFs, SMCs, and MCs, but not hepatocytes and cholangiocytes, in the adult liver. (A) A cell lineage analysis using the MesP1^Cre and R26T/G^flox mice. (B) Expression of TOMATO and GFP in the MesP1^Cre/+;R26T/G^f/f adult liver. Membrane-tagged TOMATO and GFP are observed in hepatocytes (hc) and HSCs (hsc) in the sinusoid, respectively. No GFP expression in hepatocytes (2,000 hepatocytes examined, n=3 mice). (C–I) Immunohistochemistry of the adult MesP1^Cre/+;R26T/G^f/f mouse liver for GFP with DES (C), PDPN (D), ELN (E), ACTA2 (F), CDH1 (G), HNF4 (H), or CK19 (I). Arrows indicate GFP+ HSCs in the sinusoid. Arrowheads and double arrows indicate DES+ ELN+ PFs adjacent to the bile duct (bd) and GFP+ MCs on the liver surface, respectively. Double arrowheads indicate DES+ ACTA2+ SMCs in the portal vein (pv) and hepatic artery (ha). GFP+ endothelial cells in the portal vein and hepatic artery are indicated by asterisks. No GFP expression in CDH+ HNF4+ hepatocytes and CDH+ CK19+ cholangiocytes (2,049 CDH1+, 1,836 HNF4+ hepatocytes and 1,036 CDH1+, 1,093 CK19+ cholangiocytes examined, n=3). Nuclei were counterstained with DAPI. Scale bar is 10 µm.