Fig. 2.

In vitro activation of mesodermal HSCs. HSCs were isolated from the normal MesP1^Cre/+ or MesP1⁺⁺;R26T/G^f/f adult livers and were cultured for 6 (A) and 7 days (B–D). (A) Expression of TOMATO and GFP in HSCs. Asterisks indicate GFP+ HSCs isolated from the MesP1^Cre/+;R26T/G^f/f adult liver (Cre+). No GFP expression in HSCs from the MesP1⁺⁺;R26T/G^f/f liver (Cre−). (B) Immunostaining of GFP and ACTA2 or DES in HSCs isolated from the MesP1^Cre/+;R26T/G^f/f adult liver (Cre+). Asterisks indicate GFP+ HSCs expressing ACTA2 or DES. Arrows indicate GFP− HSCs expressing ACTA2 or DES. (C) A negative control without primary antibodies. (D) Immunostaining of GFP and DES. No GFP expression in HSCs isolated from the MesP1⁺⁺;R26T/G^f/f liver (Cre−). Nuclei were counterstained with DAPI.