Fig. 3.

Similar phenotypes of GFP+ and GFP− HSCs in the MesP1^Cre/+;R26T/G^f/f liver. (A) HSCs were isolated from the MesP1^Cre/+;R26T/G^f/f (Cre+) or MesP1^+/+;R26T/G^f/f (Cre−) livers and were subjected to FACS. HSCs were analyzed by storage of VitA (FL5) and expression of GFP (FL1). (B) HSCs were isolated from the Cre+ and Cre− mice, cultured for 13 days, and subjected to FACS analysis. (C,D) HSCs were isolated from the MesP1^Cre/+;R26T/G^f/f liver and the primary (day 0) and cultured HSCs (day 13) were subjected to QPCR. In addition, VitA+GFP+ and VitA+GFP− HSCs sorted from the primary or day-13 HSCs were also subjected to QPCR for measurement for HSC markers (C) and other liver cell types (D). The results are expressed as relative expression compared against primary HSCs (day 0). The values were normalized against Gapdh. Each value is the mean ± standard deviation of the triplicate measurements.