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. 2014 May 18;13:184. doi: 10.1186/1475-2875-13-184

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Copyright © 2014 Zipprer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Conserved parasite requirement for extracellular Ca⁺⁺. (A) Normalized growth of divergent parasite lines over 72 hours as a function of measured free external [Ca⁺⁺], achieved by EGTA addition to standard media. Absissca is shown on log scale. Symbols represent mean ± S.E.M. growth of HB3, 3D7A, and Dd2 lines (green, white, and blue circles, respectively). Solid line represents the best fit to a sigmoidal decay. (B) Mean ± S.E.M normalized growth of each line when 2 mM CaCl₂ is added to the medium with 2 mM EGTA, the highest used in panel (A).