Table 1.

Strains used in this study

Name	Genotype	Additional information
Wild type	Wild-type Synechocystis	D. Bhaya (Stanford)
SAA005	PpsbA2::psbA2:: Ll ldh na	[14]
SAA024	PrnpB:: Ll ldh na	[14]
SAA025	Ptrc1:: Ll ldh na	[14]; Ptrc1 is one trc promoter with one operator sequence [18]; native version of L. lactis ldh
SAA023	Ptrc1:: Ll ldh co	[14]; codon optimized version of L. lactis ldh
SAW001	Ptrc2:: Ll ldh co	Ptrc2 is one trc promoter flanked by the operator sequence [18]
SAA026	Ptrc1:: Ll ldh co ::ldh na	Double ldh gene dosage on genome
SAW035	pDF_LDH	Expression from plasmid
SAW039	Ptrc1:: Ll ldh co , pDF_LDH	Combined expression from genome and plasmid
SYW001	Ptrc2:: Syn pk co ::Ptrc2:: Ll ldh co	pk from Synechocystis
SYW003	Ptrc2:: Ef pk co ::Ptrc2:: Ll ldh co	pk from E. faecalis
SYW004	Ptrc2:: Ec pk co ::Ptrc2:: Ll ldh co	pk from E. coli
SYW005	Ptrc2:: Ll pk co ::Ptrc2:: Ll ldh co	pk from L. lactis
SAW041	Ptrc2:: Ef pk co ::Ptrc2:: Ll ldh co , pDF_LDH	SYW003 additionally carrying pDF_LDH
SAW033	Ptrc1:: Ll ldh co , partialΔ::ppc	Partially (stable) segregated strain of the ppc knockout attempt
SAA015	Ptrc1:: Bs ldh na	[10]; native version of ldh from B. subtilis
SAA027	Ptrc1:: Bs ldh co WT	Codon optimized version of ldh from B. subtilis
SAA028	Ptrc1:: Bs ldh co V38R	V38R mutant of the codon optimized ldh from B. subtilis
SAA029	Ptrc1:: Cg ldh co WT	Codon optimized ldh from C. gunnari
SAA030	Ptrc1:: Cg ldh co V53R, E232M	V53R and E232M mutant of the codon optimized ldh from C. gunnari
SAV001	Ptrc1:: Ll ldh co L39R	L39R mutant of the codon optimized ldh from L. lactis
SAA035	Ptrc1:: Po ldh co I29R	I29R mutant of the codon optimized ldh from P. ovale