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. 2014 Jun 24;7:387. doi: 10.1186/1756-0500-7-387

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Copyright © 2014 Boggs et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Second level of stringency – extraction with Triton X-100 and NP40 – cortactin blot. MBP and cortactin co-immunoprecipitate from the OLG Triton X-100 plus NP40-insoluble pellet and supernatant fractions. Lane 1, supernatant fraction; lane 2, blank; lane 3, pellet; lane 4, anti-MBP (SMI 99) immunoprecipitate of supernatant; lane 5, control antibody immunoprecipitate of supernatant; lane 6, blank; lane 7, anti-MBP (SMI 99) immunoprecipitate of pellet; lane 8, control antibody immunoprecipitate of pellet; lane 9, anti-MBP SMI 99 IgG; lane 10, control antibody IgG. The supernatant and pellet fractions were run on a different gel from the immunoprecipitated samples. The blots were stained with rabbit polyclonal anti-cortactin antibody. Two bands at about 78 kDa and 82 kDa were detected for cortactin. Representative results are shown from 2 experiments.