Extended Data Figure 6. Motion correction improves the quality of images collected on Polora TF30 microscope using a K2 Summit direct electron detector.
a, Fourier transform of a representative cryo-EM image of TRPV1 embedded in a thin layer of vitreous ice over Quantifoil hole without supporting carbon film before motion correction. b, Path of motion of 30 individual subframes, determined as described in Methods. c, d, A nearly perfect Fourier transform (c) was restored after the EM image was corrected for motion (d).