Fig. 4.

The effect of maternal undernutrition on the expression of bile salt export pump (BSEP) gene in rat livers. (A) BSEP mRNA levels in both groups were determinated by quantitative real-time PCR, and results were expressed relative to the mean value of the E20 control. (B) and (C) Representative Western blotting and densitometric analysis of BSEP protein expression in the livers of both groups. BSEP protein expression level was normalized relative to the expression of β-actin. Data are presented as means ± SE for 6 observations per group. Black bars, control group; white bars, IUGR group. Significant differences compared with controls of same age are indicated by *p<0.05 and **p<0.01.