Fig. 1.

Citrulline increases expression of ABCA1 and ABCG1 in macrophages. A, B, THP-1 macrophages were treated with control (Cont) or 0.01, 0.1, 1 mM of citrulline (Cit) for 6 h. RNA extraction and quantitative real-time PCR were performed as described in Materials and Methods. The results from 3 separately performed experiments are expressed relative to the vehicles and presented as the mean ± SEM. Within each treatmentgroup, means without a common letter, p<0.05. C, D, THP-1 macrophages were treated with Cont or 1 mM of Cit with or without 5 mM of L-NAME for 6 h. The results from 3 separately performed experiments are expressed relative to Cont and presented as the mean ± SEM. *Different from Cont, p<0.05. RNA extraction and quantitative real-time PCR were performed as described in Materials and Methods. E, THP-1 macrophages were lysed and subjected to Western blot analysis 24 h after treatment with Cont or the indicated doses of Cit. The results are from 3 separately performed experiments that yielded similar results.