Table 1.
Sequence of oligonucleotide primers for quantitative RT-PCR analysis
| Gene name | Size (bp) | Accession No. | Primer sequence |
|
|---|---|---|---|---|
| Forward | Reverse | |||
| ChREBP | 113 | AB074517 | 5'-CAGCTTCTCGACTTGGACTG-3' | 5'-TTGCCAACATAAGCGTCTTC-3' |
| Elovl5 | 150 | NM_134382 | 5'-TTCTTCTGTCAGGGAACACG-3' | 5'-GTGCAGGACTGTGATCTGGT-3' |
| D5D | 178 | NM_053445 | 5'-AAGCACATGCCATACAACCA-3' | 5'-CAGCGGCATGTAAGTGATGA-3' |
| D6D | 144 | NM_031344 | 5'-ACATCATCGTCATGGAAAGC-3' | 5'-CAGAAAGGTGGCCATAATCA-3' |
| ATGL | 148 | NM_001108509 | 5'-GAGATGTGCAAACAGGGCTA-3' | 5'-CAGTCCTCTCCTCAGTCACG-3' |
| DGAT | 150 | NM_053437 | 5'-GACAGCGGTTTCAGCAATTA-3' | 5'-GGGGTCCTTCAGAAACAGAG-3' |
| FATP | 100 | U89529 | 5'-AGGTGACGTGCTAGTGATGG-3' | 5'-CTCCGTGGTGGATACGTTCT-3' |
| GPAT | 116 | NM_017274 | 5'-GTTATCAGAATGCTGCGGAA-3' | 5'-GAGAGGGGAGCAGATACAGG-3' |
| FAT/CD36 | 145 | NM_031561 | 5'-ATGCCGGTTGGAGACCTACT-3' | 5'-CCTCTGGGTTTTGCACGTCA-3' |
| FABP | 174 | NM_024162 | 5'-CATGAAGTCACTCGGTGTGG-3' | 5'-TCATCTGCTGTGACCTCGTC-3' |
| Elovl6 | 99 | NM_134383 | 5'-AGCAAAGCACCCGAACTAGG-3' | 5'-GTACAGGAGCACAGTGATGTGG-3' |
| 5-LOX | 198 | NM_012822 | 5'-CCCATCTGCCTGCTGTATAA-3' | 5'-CAGATGTGTGCGGAGAAGAT-3' |
| 12-LOX | 92 | L06040 | 5'-TCTGGCAGATCATGAATCGG-3' | 5'-GCTCTGCAGTTCATAGTCGT-3' |
| PPARγ | 147 | AF156665 | 5'-TCAAACCCTTTACCACGGTT-3' | 5'-CAGGCTCTACTTTGATCGCA-3' |
| TNF-α | 143 | NM_012675 | 5'-ATGGATCTCAAGACAACCA-3' | 5'-TCCTGGTATGAAATGGCAAA-3' |
| MCP-1 | 93 | M57441 | 5'-CCCTAAGGACTTCAGCACCTTTG-3' | 5'-AAGTGCTTGAGGTGGTTGTGG-3' |
| Acrp30 | 140 | NM_144744 | 5'-GGAAACTTGTGCAAGGTTGGA-3' | 5'-GGTCACCCTTAGGACCAAGA-3' |
| Bip | 279 | NM_013083 | 5'-CCTGTTGCTGGACTCTGTGA-3' | 5'-AGGAGTGAAGGCCACATACG-3' |
| Chop | 231 | NM_001109986 | 5'-TTGGGGGCACCTATATCTCA-3' | 5'-CGCACTGACCACTCTGTTTC-3' |
| Xbp1 | 229 | NM_001004210 | 5'-GAGCAGCAAGTGGTGGATTT-3' | 5'-GAGGCGCACGTAGTCTGAGT-3' |
| Atf3 | 225 | NM_012912 | 5'-CCAGGTCTCTGCCTCAGAAG-3' | 5'-GCCACCTCAGACTTGGTGA-3' |
| Atf6 | 249 | NM_001107196 | 5'-AGCAGAGCCACTGAAGGAAG-3' | 5'-GGGGTCCTTCAGAAACAGAG-3' |
| LPCAT3 | 188 | NM_001012189 | 5'-GATGGCCTACCTTCTTGCTG-3' | 5'-ATGAGGGGACACCCAGTATG-3' |
| LPIAT1 | 177 | NM_001134978 | 5'-ATTGCAGCTTCCCTGGAGTA-3' | 5'-GGCACTCCTCAAAACGTAGG-3' |
| SERCA1 | 170 | NM_058213 | 5'-AAAGGAAGGAGCCCAGGTTA-3' | 5'-GCTCACCCCAAAATAGGACA-3' |
| SERCA2a | 228 | NM_001110823 | 5'-TATTGGCTGGTGAAGGAGGT-3' | 5'-GACAATGTCTGCTGGCTCAA-3' |
| SERCA2b | 176 | NM_001110139 | 5'-CCGCTTCCTAAACATTGCAG-3' | 5'-CAACAGCTAAAACCCCACTTG-3' |