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. Author manuscript; available in PMC: 2015 Jun 11.
Published in final edited form as: Cell Host Microbe. 2014 Jun 11;15(6):729–740. doi: 10.1016/j.chom.2014.05.009

Figure 1. Schematic Representation of the GAC Gene Cluster, Mutagenesis Scan, and Δ GacI mutant.

Figure 1

(A) Schematic representation of the GAS M1T1 strain 5448 group A carbohydrate (GAC) gene cluster M5005_Spy0602-0613, which was renamed gacA-L, and annotated gene functions based on analysis using the SEED tools (URL: pubseed.theseed.org). (B)Latex agglutination reaction with GAC-specific beads and GlcNAc-specific sWGAlectin staining of viable GAS inserional knockout mutants in genes gacD-L. Grey fill: medium control; black line:sWGA-FITC stain. Histogram numbers indicate geometric mean of fluorescence. (C) Latex agglutination reaction with GAC-specific beads and GlcNAc expression as assessed by sWGA stain as indicated on GAS WT, ΔGacImutant and GacI* complemented strain. HPLC tracing and linkage analysis with deduced schematic structure of the repeating unit of extracted GAC from (D) GAS WT and (E)ΔGacI mutant strain. See also Figure S1.