Aversive prediction error signals in amygdala TO2 signals during fear learning. A, Freezing responses during aversive conditioned (CS+) or nonaversive conditioned (CS−) cues for training days 1–3 (T1–T3). Freezing is plotted as a difference score (freezing during the 30 s of cue presentation minus freezing in the 30 s before cue presentation), with negative scores indicating less freezing during the cue compared with the 30 s before the cue. B, Amygdala TO2 responses during the 30 s CS+ period and the 30 s post-US and the CS− and the equivalent 30 s no US period for training days 1–3. C, Peak TO2 responses during CS+ and US periods, and CS− and no US periods, during training days 1–3. A–C, Mean ± SEM (n = 24 mice); *p < 0.05.