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. 2014 Jul 2;34(27):9124–9133. doi: 10.1523/JNEUROSCI.0181-14.2014

Figure 6.

Figure 6.

Effect of PPN lesion on sleep in three MPTP-treated macaques. A, Photomicrographs of PPN sections labeled for NADPH histochemistry, showing the toxin injection site into the PPN (arrow) in the left (L) side of Macaque 2 and in both sides of Macaque 3 compared with the right (R) unlesioned side of Macaque 2. B, Left, NADPH+ neurons (blue) were mapped in two lesioned animals. Each dot represents an NADPH+ neuron. Right, Quantification of the total number of NADPH+ neurons in the PPN showing the neuronal loss for unilaterally lesioned macaques (Macaques 2 and 4), and for bilaterally lesioned macaque (Macaque 3). C, Values of relevant sleep parameters in the three macaques 1–3 weeks after PPN lesion compared with control (CTL) and MPTP values. Note a decrease of sleep efficiency, REM sleep and SWS, and an increase of nighttime awakenings (wake). These disorders were much more severe in Macaque 2 than in Macaques 3 and 4. These parameters progressively returned to values observed after MPTP treatment, except for Macaque 2, which was therefore killed 3 weeks after PPN lesion. Scale bar: A, 1 mm.