A, Four prostate tissue samples from transition zones were collected to extract whole cell proteins. Protein extracts (100 μg) were separated on a protein gel together with 5 μg of protein lysates from T47D cells and Western blotted with PR antibody. Information on these 4 tissue samples is also described. B, Whole-mount sections of human prostate biopsies (n = 27) were stained with PRB-specific antibody (C1A2). Four regions within the section (ID no. 7219) were amplified: peripheral zone (benign prostate), peripheral zone (cancer prostate), transitional zone, and ejaculatory duct. LT, left side; RT, right side.