A whole-mount section of human prostate tissue (ID no. 7219) was costained with PR and α-SMA antibodies by IHC (A) or by immunofluorescence (B). In IHC studies, PR antibody was detected by UltraMap Anti-Rb HRP, whereas α-SMA antibody was detected by UltraMap Anti-Ms Alk Phos (Ventana Medical Systems, Oro Valley, Arizona). In immunofluorescence studies, PR antibody was detected by Alexa Fluor 568 (Invitrogen, San Diego, California), whereas α-SMA antibody was detected by Alexa Fluor 488 (Invitrogen).