Table 2. Targeted nucleotide repair is elevated by over-expression of RAD51 and RAD54.
| Strainsa | Hygr | Aurr (×105) | CE (×10–5)b | Fold |
|---|---|---|---|---|
| LSY678 (Int) pYN132+pYNU132 | 1249 ± 55 | 111 ± 12 | 12.39 ± 1.10 | 1 |
| LSY678 (Int) pYNRAD51+pYNU132 | 7134 ± 445 | 133 ± 11 | 53.60 ± 5.29 | 4.3 |
| LSY678 (Int) pYNRAD54+pYNU132 | 3372 ± 409 | 128 ± 35 | 26.18 ± 8.68 | 2.1 |
| LSY678 (Int) pYNRAD51+pYNURAD54 | 44 008 ± 5064 | 87 ± 18 | 488.01 ± 191.38c | 39.4 |
| LSY678 (Int) pYNRAD51+pYNUSGS1 | 7121 ± 459 | 98 ± 16 | 74.36 ± 9.83 | 6.0 |
| LSY678 (Int) pYN132+pYNUSGS1 | 1498 ± 109 | 141 ± 21 | 10.94 ± 1.38 | 0.9 |
aLSY678(Int) with a combination of pYN132 and pYNU132 expression vectors were targeted with Hyg3S/74NT and plated on Hyg-agar and Aur-agar plates as described in Materials and Methods.
bHygromycin- or aureobasidin- resistant colonies, as well as the correction efficiency from five experiments, are presented as mean and standard deviation. Statistical analysis was done by ANOVA test at SAS program.
cCompared to that of LSY678(Int) pYN132+pYNU132, P < 0.05 by ANOVA test.