Figure 2.

Variation of fluorescence emission intensity as a function of time for duplex 4 (i.e. having 2-AP at the position of CH₃ addition and ^MeA on the complementary oligodeoxynucleotide, Table 1) in the presence of M.EcoKI. The reaction is initiated by the addition of AdoMet and the formation of 2-AP* (open circle, emission at 430 nm) and the decay of 2-AP (filled circle, emission at 370 nm) observed. A first-order rate equation was used to fit the data and the reaction was essentially complete after 5 min although the spectra shown in other figures were obtained after 15 min of reaction.