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Tritium assay of methylation of different 2-AP-containing duplexes by the wild-type M.EcoKI showing the amount of radioactivity incorporated into the DNA during a 30 min incubation with [3H-methyl]AdoMet. Lanes 1 to 4 are duplexes 3, 1, 4 and 2, respectively. Lanes 5 and 6 are the parent duplex (Table 1) in fully un-methylated and hemi-methylated forms, respectively.
