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Nuclear halos immunolabeled with an antibody against lamin A (A′) or hybridized in situ with an X chromosome-specific alphoid satellite probe (alphX) (B′, C′). (A–C) DNA stained with DAPI. (D) Superposition of DAPI staining and the alphX hybridization signal; the DAPI image is discriminated into 20 intensity levels in order to facilitate precise positioning of the alphX signal.
